Table of Contents Author Guidelines Submit a Manuscript
International Journal of Dentistry
Volume 2016, Article ID 8351427, 6 pages
http://dx.doi.org/10.1155/2016/8351427
Clinical Study

Nitric Oxide Concentration and Other Salivary Changes after Insertion of New Complete Dentures in Edentulous Subjects

1Department of Biochemistry and Molecular Biology, Biological Sciences Institute, Federal University of Goias, Avenida Universitária Esquina com 1a Avenida s/n, Setor Universitário, 74605-220 Goiania, GO, Brazil
2Department of Oral Prevention and Rehabilitation, School of Dentistry, Federal University of Goias, Avenida Universitária Esquina com 1a Avenida s/n, Setor Universitário, 74605-220 Goiania, GO, Brazil

Received 4 November 2015; Accepted 7 February 2016

Academic Editor: Manal Awad

Copyright © 2016 Maria de Lourdes Breseghelo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To assess changes in levels of salivary nitric oxide (NO) after insertion of new complete dentures and its association with clinical and salivary parameters. Methods. Nineteen fully edentulous subjects were included, mean age 64.4. Unstimulated whole saliva was collected before and after insertion of the dentures, at follow-up visits, and after 12 months. The concentration of the final stable NO product (nitrite) was measured by a colorimetric assay based on the Griess reaction. Clinical parameters were assessed during all clinical visits. Results. Functional adaptation to the dentures progressively improved, with no complaints at the long-term follow-up. NO concentration was not influenced by the level of functional adaptation, presence of injuries to the mucosa, salivary flow, and saliva viscosity. Pairwise comparison showed a reduction in NO concentration at the first follow-up compared to baseline values but differences were not statistically significant. Significant differences were observed in NO concentrations at the long-term follow-up when compared to the first () and second () visits. Conclusion. NO concentration reduced after denture insertion and returned to baseline levels in the long-term follow-up. This appears to be an autonomic response of the body and provides valuable complementary information for the management of the edentulous patient.