Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage

Figure 6

Adjustment of LCM parameters allows capturing of different sizes/areas of tissue. (a) MCC tissue section is prepared for LCM. (b) The section after LCM, where clusters of cells removed. LCM power and duration were adjusted to avoid burning (arrow) of the cap film. (c) Proper wetting permits the film to contact the selected area of the tissue section. (d) The section after LCM demonstrating capturing of a strip (continuous line) of tissue and (e) capturing of a larger zone. (f) After LCM completion, the LCM cap is examined to confirm successful microdissection of the cells of interest (scale bar = 100 μm).

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