Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage

Table 1

Sequence of primer sets for housekeeping genes used for qRT-PCR to evaluate LCM-RNA integrity.

Gene symbol	Forward primer sequence (5′-3′)	Reverse primer sequence (5′-3′)
GAPDH (3′ end)	ATGTATCCGTTGTGGATCTGACAT	AGCCCAGGATGCCCTTTAGT
GAPDH (midregion)	CCTCAAGATTGTCAGCAATGCA	GGCATGGACTGTGGTCATGA
β-Actin (3′ end)	GCTCCTCCTGAGCGCAAGT	CATCGTACTCCTGCTTGCTGAT
β-Actin (midregion)	GGCCAACCGTGAAAAGATGA	ACCAGAGGCATACAGGGACAA