Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Group</td><td class="align_center">3′/m GAPDH (mean ± SD)</td><td class="align_center">3′/m <i>β</i>-Actin (mean ± SD)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">C (femoral condylar cartilage)</td><td class="align_center">1.11 ± 0.11</td><td class="align_center">1.41 ± 0.24</td></tr><tr><td class="align_left">FZ (fibrous zone of MCC)</td><td class="align_center">1.28 ± 0.21</td><td class="align_center">1.93 ± 0.46</td></tr><tr><td class="align_left">PZ (proliferative zone of MCC)</td><td class="align_center">1.43 ± 0.23</td><td class="align_center">1.88 ± 0.28</td></tr><tr><td class="align_left">MZ (mature zone of MCC)</td><td class="align_center">1.56 ± 0.26</td><td class="align_center">2.12 ± 0.41</td></tr><tr><td class="align_left">HZ (hypertrophic zone of MCC)</td><td class="align_center">1.44 ± 0.15</td><td class="align_center">2.04 ± 0.29</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Average values of 3′/m GAPDH and <i>β</i>-Actin ratio for LCM-RNA samples retrieved from groups C (the control), FZ, PZ, MZ, and HZ.</div>

International Journal of Dentistry

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Table 2

Table 2: Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage 