Assessment of Cytokine Release against Oral Mucosal Cell Line Culture (TR146) Stimulated by Neutrophil Elastase Associated with Behcet’s Disease

Table 3

Detection of TNF-α following the treatment of the OMM with NE after 24 hours of incubation.

Parameters	NE 10 nM	NE 50 nM	NE 100 nM	NE 200 nM	Negative control (saline)	Positive control (SLS 1%)	P ≤ 0.05
TNF-α (pg./ml) 24 hours of incubation	—	—	14.76 ± 1.72	23.23 ± 3.71	22.26 ± 4.09	141.84 ± 51.66	ns/ns
TNF-α (pg./ml) 4 hours of incubation	2.61 ± 1.43	25.00 ± 55.90	1.17 ± 0.50	4.36 ± 4.86	2.62 ± 1.64	± 3.16	ns/ns

; ; . ns = nonsignificant, a/a† = compared with the negative control/compared the positive control, b/b† = compared the negative control with 10 nM NE/compared with the positive control with 10 nM NE, c/c† = compared the negative control with 50 nM NE/compared the positive control with 50 nM NE, d/d† = compared the negative control with 100 nM NE/compared the positive control with 100 nM NE, e/e† = compared the negative control with 200 nM NE/compared the positive control with 200 nM NE, g/g† = compared 10 nM NE with 100 nM NE (negative control group)/compared 10 nM NE with 100 nM NE (positive control group), and i/i† = compared 50 nM NE with 100 nM NE (negative control group)/compared 50 nM NE with 100 nM NE (positive control group).