Interleukin-1 Gene Variability and Plasma Levels in Czech Patients with Chronic Periodontitis and Diabetes Mellitus

Table 2

IL-1 genotype and allele frequencies in patients with CP, T1DM (and T1DM + CP subgroup), T2DM (and T2DM + CP subgroup), and healthy controls.

Genotypes alleles	Controls ()	CP ()	OR (95% CI)	T1DM ()	OR (95% CI)	T1DM + CP ()	OR (95% CI)	T2DM ()	OR (95%CI)	T2DM + CP ()	OR (95% CI)
IL-1A (−889C/T)	(%)	(%)		(%)		(%)		(%)		(%)
CC	105 (46.7)	145 (54.9)	1.00	61 (46.2)	1.00	20 (52.6)	1.00	198(50.1)	1.00	46 (51.7)	1.00
CT	102 (45.3)	104 (39.4)	0.74 (0.51–1.07)	62 (47.0)	1.05 (0.67–1.63)	15 (39.5)	0.77 (0.37–1.59)	160 (40.5)	0.83 (0.59–1.17)	34 (38.2)	0.76 (0.45–1.28)
TT	18 (8.0)	15 (5.7)	0.70 (0.34–1.36)	9 (6.8)	0.86 (0.36–2.03)	3 (7.9)	0.88 (0.24–3.25)	37 (9.4)	1.09 (0.59–2.01)	9 (10.1)	1.14 (0.48–2.73)
C allele	312 (69.3)	394 (74.6)	1.00	184 (69.7)	1.00	55 (72,4)	1.00	556 (70.4)	1.00	126 (70.8)	1.00
T allele	138 (30.7)	134 (25.4)	0.77 (0.58–1.02)	80 (30.3)	0.98 (0.71–1.37)	21 (27.6)	0.86 (0.50–1.48)	234 (29.6)	0.95 (0.74–1.22)	52 (29.2)	0.93 (0.64–1.36)
IL-1B (+3953C/T)
CC	115 (51.1)	164 (62.1)	1.00	73 (55.3)	1.00	18 (47.4)	1.00	220 (55.7)	1.00	50 (56.2)	1.00
CT	94 (41.8)	87 (33.0)	0.65(0.45–0.95)	48 (36.4)	0.80 (0.51–1.27)	17 (44.7)	1.16 (0.56–2.37)	143 (36.2)	0.80 (0.56–1.12)	30 (33.7)	0.73 (0.43–1.25)
TT	16 (7.1)	13 (4.9)	0.57 (0.26–1.23)	11 (8.3)	1.08 (0.48–2.46)	3 (7.9)	1.20 (0.32–4.53)	32 (8.1)	1.05 (0.55–1.98)	9 (10.1)	1.29 (0.54–3.12)
C allele	324 (72.0)	415 (78.6)	1.00	194 (73.5)	1.00	53 (69.7)	1.00	584 (73.8)	1.00	130 (73.0)	1.00
T allele	126 (28.0)	113 (21.4)	0.70 (0.52–0.94)	70 (26.5)	0.93 (0.66–1.31)	23 (30.3)	1.12 (0.66–1.90)	206 (26.2)	0.91 (0.70–1.18)	48 (27.0)	0.95 (0.64–1.40)
IL-1RN (VNTR)^§
11	108 (48.0)	121 (45.8)	1.00	83 (62.9)	1.00	19 (50.0)	1.00	220 (55.7)	1.00	48 (53.9)	1.00
12	87 (38.7)	110 (41.7)	1.13 (0.77–1.65)	31 (23.5)	0.46 (0.28–0.76)	11 (28.9)	0.72 (0.32–1.59)	129 (32.7)	0.73 (0.51–1.04)	25 (28.1)	0.65 (0.37–1.13)
13	8 (3.6)	1 (0.4)	0.11 (0.01–0.91)	1 (0.8)	0.16 (0.02–1.33)	1 (2.6)	0.71 (0.08–6.01)	3 (0.8)	0.18 (0.05–0.71)	1 (1.1)	0.28 (0.03–2.31)
14	3 (1.3)	8 (3.0)	2.38 (0.62–9.20)	4 (3.0)	1.73 (0.38–7.96)	1 (2.6)	1.89 (0.19–19.19)	9 (2.3)	1.47 (0.39–5.55	4 (4.5)	3.00 (0.65–13.93)
22	17 (7.6)	17 (6.4)	0.89 (0.43–1.83)	8 (6.1)	0.61 (0.25–1.49)	2 (5.3)	0.67 (0.14–3.13)	31 (7.8)	0.90 (0.47–1.69)	9 (10.1)	1.19 (0.50–2.86)
23	0	1 (0.4)	#	1 (0.8)	#	1 (2.6)	#	0	#	0	#
24	2 (0.9)	5 (1.9)	2.23 (0.42–11.74)	3 (2.3)	1.95 (0.32–11.95)	2 (5.3)	5.68 (0.75–42.84)	3 (0.8)	0.74 (0.12–4.47)	2 (2.2)	1.38 (0.19–10.04)
44	0	0	#	1 (0.8)	#	1 (2.6)	#	0	#	0	#
16	0	1 (0.4) ^§§	#	0	#	0	#	0	#	0	#
L allele	327 (72.7)	376 (71.5)	1.00	213 (80.7)	1.00	58 (76.3)	1.00	596 (75.4)	1.00	133 (74.7)	1.00
S allele	123 (27.3)	150 (28.5)	1.06 (0.80–1.40)	51 (19.3)	0.64 (0.44–0.92)	18 (23.7)	0.83 (0.47–1.46)	194 (24.6)	0.87 (0.67–1.13)	45 (25.3)	0.90 (0.61–1.34)

CI = confidence interval; CP = chronic periodontitis; OR = odds ratio; T1DM = type 1 diabetes mellitus; T2DM = type 2 diabetes mellitus. in comparison to healthy controls (evaluated by Fisher’s exact test, without correction for multiple comparisons). ^#Cannot be assessed because of the small number. ^§Alleles with more than two repeat units were grouped together as IL-1RN ^∗ L (“long” allele) and 2 repeat units were marked as IL-1RNS (“short” allele). ^§§Sample with rare genotype IL-1RN16 was excluded from allele frequencies and haplotype analysis.