A New Controlled-Release Material Containing Metronidazole and Doxycycline for the Treatment of Periodontal and Peri-Implant Diseases: Formulation and In Vitro Testing

Table 1

Zones of microbial inhibition induced by Met/Dox gel (20 mg/ml and 10 mg/ml, respectively) on microaerophilic cultures (Aggregatibacter actinomycetemcomitans) or in anaerobic conditions (Streptococcus sanguinis, Parvimonas micra, and Eikenella corrodens) with respect to a negative placebo control = 0 mm for all strains, for all observational intervals.

Inhibition zones (mm)
Time (hours)	A. actinomycetemcomitans	S. sanguinis	P. micra	E. corrodens
24	40 ± 3	40 ± 2	40 ± 3	40 ± 4
48	38 ± 2	40 ± 4	40 ± 4	37 ± 3
72	36 ± 1	35 ± 2	35 ± 4	35 ± 2
96	25 ± 2	30 ± 3	32 ± 2	30 ± 2
120	25 ± 1	30 ± 2	30 ± 1	27 ± 3
144	20 ± 2	27 ± 1	28 ± 2	25 ± 3
168	15 ± 1	25 ± 2	25 ± 1	25 ± 1
240	14 ± 1	15 ± 3	17 ± 1	15 ± 3
312	12 ± 1	11 ± 2	10 ± 1	10 ± 2

Data are expressed as mean inhibition zone (mm) ± SD of three replicates.