Polymodal Activation and Desensitization of TRPV1 Receptor in Human Odontoblasts-Like Cells with Eugenol

Figure 2

Immunocytochemical detection of odontogenic markers DMP-1 and DSP in hOLCs. Positive immunostaining (green) of tissue control (a) mammary gland for DMP-1 and (b) placenta for DSP. Positive staining of dental tissue in the odontoblast layer and mineralization in front of the dentin. To determine the presence and location of the odontogenic markers in the differentiated cells, an anti-DMP-1 (a) or anti-DSP (b) antibody conjugated with FITC (green) was used, and the nuclei were counterstained with Hoechst stain (blue). Note the positivity of these markers in hOLCs, with both a nuclear and cytoplasmic distribution. A Zeiss Axio Imager A2 microscope was used to obtain the images. Scale bar: 50 µm.

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