Polymodal Activation and Desensitization of TRPV1 Receptor in Human Odontoblasts-Like Cells with Eugenol

Figure 6

Chemical, thermal, and pH stimuli of TRPV1 receptor in hOLCs. In the polymodal stimulation assays, 1 and 20 min of exposure to the stimulus were evaluated using the Fluo 4 AM probe. (a) Chemical stimulation of the TRPV1 receptor. Statistically significant differences were found between the test groups at the different exposure times evaluated (1 and 20 min) with both CAP and EUG. TRPV1 receptor desensitization was confirmed at 20 min with both vanilloid substances, and no differences from the control were identified. (b) Thermal stimulation activation at 43°C. No statistically significant differences were found between the test groups at the different exposure times evaluated (1 and 20 min). (c) Stimulation with changes in pH. Activation was marked at pH 5 and 6. Relative fluorescence units (RFU) were detected at a wavelength of 494/520 nm (excitation/emission) using a TECAN microplate reader. Results are shown as the mean ± SD of three independent experiments in triplicate (n = 9). Each group was compared to its respective control, and the asterisks show statistically significant differences between them (). The lowercase letter (a) shows statistically significant differences between the test groups at the different times evaluated (1 and 20 min) ().

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