Research Article
The Impact of DNA Methylation on IL6 mRNA Levels in Hematinic Deficiency and Atopy-Associated Recurrent Aphthous Stomatitis Patients
Figure 2
(a) The cycle threshold (CT) of the IL6 gene in the RAS group. (b) The CT of the IL6 gene in the control group (c) Quantitation data for Cycling A.Green. The result and data from RT PCR are presented as cycle threshold (CT), and (Figures 1–3) the CT is the cycle in which the fluorescence level reaches a specified amount (the threshold). This method (2−ΔΔCT formula) [41] immediately uses the CT information, and the end result is normalized as a change in the expression of the target gene (IL6) in the target sample (RAS) compared to a shown reference sample (control) to a reference gene (GADPH).
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