Desquamated Epithelial Cells of Unstimulated Human Whole Saliva Express Both EGF Transcript and Protein

Table 1

Ct values for the epidermal growth factor (EGF), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA (18S) in negative controls (RNAse-free water), and RNA samples for DOECs and HaCaT cells. Gene expression was assessed in DOECs isolated from all 4 individuals included in the study. Values are presented as means ± SEM for three experiments. ND: not detected.

Sample	Ct value EGF	Ct value GAPDH	Ct value 18S
DOEC	32.70 ± 0.84	26.20 ± 0.59	11.69 ± 0.81
HaCaT	28.85 ± 0.30	20.76 ± 0.49	5.57 ± 0.18
Negative control	ND	ND	27.92 ± 0.11