Probiotics-Containing Mucoadhesive Gel for Targeting the Dysbiosis Associated with Periodontal Diseases
Table 6
Lacto-Counter Assay (LCA) enumeration of lactobacilli contained in the lipogels AL0006 and AL0007.
Time (months)
T0
30 min
2 h
5 h
8 h
Start
Gel
Solution
Gel
Solution
Gel
Solution
Gel
Solution
AL0006 lipogel preparation
T0
4.90 × 109
3.81 × 109
2.24 × 106
2.49 × 108
1.55 × 107
1.10 × 108
4.50 × 107
0.68 × 108
6.42 × 108
T3
4.00 × 109
3.70 × 109
5.53 × 106
2.14 × 108
8.63 × 106
1.84 × 108
2.00 × 107
1.13 × 108
2.00 × 108
T6
4.10 × 109
3.23 × 109
3.00 × 106
4.83 × 108
1.44 × 107
4.64 × 108
3.30 × 107
1.19 × 108
8.65 × 108
T9
2.20 × 109
1.97 × 109
6.33 × 106
1.13 × 109
7.23 × 106
6.25 × 108
3.20 × 107
5.77 × 107
7.98 × 108
T12
2.10 × 109
1.97 × 109
6.62 × 106
1.20 × 109
9.46 × 106
6.45 × 108
7.20 × 107
2.35 × 107
1.31 × 108
Time (months)
T0
30 min
2 h
5 h
8 h
1 g
1 g
1 ml PS
1 g
1 ml PS
1 g
1 ml PS
1 g
1 ml PS
AL0007 lipogel preparation
T0
3.90 × 109
3.14 × 109
1.60 × 106
2.60 × 108
1.87 × 107
1.70 × 108
3.80 × 108
1.16 × 108
7.51 × 108
T3
4.30 × 109
2.00 × 109
4.67 × 106
7.60 × 108
1.80 × 108
4.90 × 108
2.70 × 108
1.44 × 108
7.00 × 108
T6
3.80 × 109
1.32 × 109
3.56 × 106
8.04 × 108
2.49 × 107
5.46 × 108
7.34 × 108
2.41 × 108
8.56 × 108
T9
2.50 × 109
1.64 × 109
3.64 × 107
1.50 × 109
4.00 × 107
6.44 × 108
1.83 × 108
1.40 × 108
8.17 × 108
T12
2.12 × 109
1.98 × 109
2.62 × 107
1.10 × 109
8.57 × 107
5.33 × 108
3.12 × 108
9.36 × 107
1.04 × 109
Enumerations of bacteria on lipogels AL0006 (a) and AL0007 (b) and their release in physiological solution. Time T0 is referred to the quantity of lactobacilli present in the lipogels, while time points 30 min, 2, 5, 8 h refer to the quantity of bacteria remaining in the 1 g lipogel (Gel) or released in solutions (1 g/ml) (solution) after each incubation time. Bacterial load and release from lipogels to physiological solution (PS) were evaluated after 3 (T3), 6 (T6), 9 (T9), and 12 (T12) months on a shelf at room temperature. Values are reported as mean. The standard deviation (SD) ranged between 0.1 and 0.3 for all the means and was not reported.
