Equisetum arvense Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis

Figure 1

Effect of Equisetum arvense extract (EA) on lipopolysaccharide (LPS)-induced osteoclast formation. Effects of EA (15 μg/mL) on osteoclast formation by Escherichia coli (E. coli)-LPS (5 mg/mL). Periodontal tissue was collected 3 days following treatment, and immunohistochemical (IHC) staining was performed. (a) Illustration of the osteoclast counting area. (b) Immunoexpression of cathepsin K, an osteoclast marker after 3 days following (A, A′) physiological saline (control), (B, B′) E. coli-LPS, and (C, C′) E. coli-LPS/EA application. Scale bars = 100 μm. (c) The number of cathepsin K-positive osteoclasts formed along the alveolar bone margin within 1 mm of the alveolar crest is counted. Data are presented as means ± standard deviation (n = 7 for each group). Tukey–Kramer multiple comparison test, .

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