Equisetum arvense Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis

Figure 3

Effect of Equisetum arvense extract (EA) on osteoclast formation-related factors. (a) It has been reported that stimulation of osteoblasts with lipopolysaccharide (LPS) alters the expression of osteoclast formation-related factors. Therefore, we examined the effect of EA on osteoclast formation-related factors. Tumor necrosis factor-α, interleukin (IL)-6, and the receptor activator of NF-κB ligand (RANKL) mRNA expression of ST2 stimulated with Escherichia coli (E. coli)-LPS (1 μg/mL) with or without EA (3 μg/mL) for 2 hours, and total RNAs are extracted (A, B, C). IL-1β and Sema3A mRNA expression of ST2 stimulated with E. coli-LPS with or without EA for 12 hours, and total RNAs are extracted (D, F). Osteoprotegerin (OPG) mRNA expression of ST2 stimulated with E. coli-LPS with or without EA for 24 hours, and total RNAs are extracted (E). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is used as an internal control. Data are presented as means ± standard error (n = 7 for each group). Tukey–Kramer multiple comparison test, , , . (b) RANKL and OPG protein expression of ST2 stimulated with Escherichia coli (E. coli)-lipopolysaccharide (LPS) (1 μg/mL). Cell culture supernatants were collected 3 hours (for RANKL) or 48 hours (for OPG) after treatment and analyzed using an ELISA assay. Data are presented as means ± standard error (n = 6 for each group). Tukey–Kramer multiple comparison test, .

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