Evaluation of Association of Oral Bacterial Profile with HBV and HCV Infection and T Lymphocyte Level in HIV-Positive Patients

Table 1

Primers and probes for real-time PCR.

Name of bacteria	Primers and probes for real-time PCR	Annealing
S. salivarius	Forward primer: MKK-GTGTTGCCACATCTTCACTCGCTTCGG; Reverse primer: MKK- CGTTGATGTGCTTGAAAGGGCACCATT	54
S. mutans	Forward primer: 5-GGCACCACAACATTGGGAAGCTCAGTT; Reverse primer: 5-GGAATGGCCGCTAAGTCAACAGGAT	53
S. sanguinis	Forward primer: GGATAGTGGCTCAGGGCAGCCAGTT; Reverse: GAACAGTTGCTGGACTTGCTTGTC	55
Lactobacillus	Forward primer: AGAGTTTGATTGGCTCAG; Reverse: CACCGCTACACATGGAG	52
E. faecalis	Forward: 5-ATC AAG TAC AGT TAG TCT TTA G-3; Reverse:5-ACG ATT CAA AGC TAA CTG AAT CAG T-3	55
Actinomyces	5-AGCGCTTGCCTTTTTGGTG-3 (annealing 53); 5-AACCCGCCATGCGACAGACCCG-3	56
Fusobacterium periodonticum	5-ACCTTATCAAGACTTATT ATT TC-3 (annealing 55); 5-TCA AAC TCT ATY TCA GGA ACA A-3	52
Bacteroides	GTTTAATTCGATGATACGCGAG; TTAASCCGACACCTCACGG	52
Actinobacteria	TGTAGCGGTGGAATGCGCAATTAAGCCACATGCTCCGCT	55
Campylobacter gracilis	5'-AAC GGA ATT TAA GAG AGC TT-3′; 5′-CTT TCC CGA TTT ATC TTA TG	54
Genus Campylobacter	AGTCTTGGCAGTAATGCACCTAACGATATGCCATTGTAGCACGTGTGTCG	52
Peptostreptococcaceae	ACTCCTACGGGAGGCAGCAGGACTACTTGGGTATCTAAT	56
Synergistetes	GAGTACCGGAGAGGCAAGTGAGTTACCGTCCAGCAAGTCG	56
Neisseriaceae	AACATATCGGAACGTACC (probe)	50
Eubacteria	AGAGTTTGATCATGGCTCAGGCTGCTGGCACGAAGTT	54
Staphylococcus aureus	CATGGTTCAAAAGTGAAAGAC (probe)	52
Streptococcus genus	GCGGGGGATAACTATTG (probe)	52
Corynebacterium genus	CGAAGCTTTTGTGACGG (probe)	52
Enterobacter genus	AGGAAGGTGTTGTGGTTAA (probe)	55
Abiotrophia	GCGTGAATGCCATCTATCAGACTTGTTGGTCTCGCAGTCA	57
Kingella	TGCCAAAGTAAAACCAGCTGAAAACTTACCTAATTTTGGCAAAGCAA	55
Prevotella	CACTGTAAACGATGGATGCCCCAGACGTTGGGCTGGTTTA	58
Veillonella	GAAAGAAGCGCGCACCGACAGTGTGTAACAAGGGAGTACGGACC	55
Rothia	GGGTTGTAAACCTCTGTTAGCATCCGTACCCACTGCAAAACGCAG	52
Parvimonas	AGAGTTTGATCCTGGCTCAGACGGCTACCTTGTTACGACTT	50
Leptotrichia	GAGTTTGATYCTGGCTCAGAAGGAGGTGATCCAACCGCA	59
Solobacterium	AGTTTGATCCTGGCTCAGCTTGTTACGACTTCACCC	55
Gemella	AGAGTTTGATCCTGGCTCAGACGGCTACCTTGTTACGACTT	54
Haemophilus	AGAGTTTGATCTGGCTCAGGACGGGCGGTGTGTACA	50
Dialister	TTC TAA GCA TCG CAT GGT GCGAT TTC GCT TCT CTT TGT TG	57