An In Vitro Pilot Study on the Effects of Silver Diamine Fluoride on Periodontal Pathogens and Three-Dimensional Scaffolds of Human Fibroblasts and Epithelial Cells

Figure 1

Effect of SDF on bacterial growth. First row, S. mutans was streaked on brain heart infusion agar plates. SDF dilutions are annotated in bottom two quadrants of agar plates. SDF inhibited S. mutans growth at all concentrations tested. Second row, P. gingivalis was streaked on tryptic soy with 5% defibrinated sheep blood agar plates. Note the SDF precipitate artifact in 12.6% concentration lower left quadrant. Colonies growth was noted in quadrants with 0.197% and 0.098% SDF. Third row, A. actinomycetemcomitans was streaked onto brain heart infusion agar plates. Due to the need for longer incubation periods and the fast growth and spread of A. actinomycetemcomitans on agar plates, colonies could not be cultured by quadrant as with the other bacteria. Colonies were instead inoculated on separate Petri dishes. SDF inhibited A. actinomycetemcomitans growth at all concentrations tested. All concentrations were performed in triplicate, and the process was repeated twice.