An In Vitro Pilot Study on the Effects of Silver Diamine Fluoride on Periodontal Pathogens and Three-Dimensional Scaffolds of Human Fibroblasts and Epithelial Cells

Figure 3

Effect of SDF on HSE constructs. The HSE constructs used are grown on Fisher CellSafe Biopsy Inserts, which are 18 × 18 mm in dimension. (a) Control untreated construct. B through F are treated constructs that received 3 μL of 38% SDF and incubated for: (b) 12 hours: center of lesion is white-gray and reticulated with smooth surrounding tissues; (c) 24 hours: color of lesion shifts to a more grayish hue with slight contraction noted across surface of lesion and surrounding tissues; (d) 3 days: dark pigmentation starts to develop with an induration of the affected keratinized layer of epithelium; (e) 5 days: Continued darkening of pigmentation with inflammation in surrounding epithelium; and (f) 10 days: Complete induration noted with blackened crust and contraction of eschar from underlying tissue (white arrow).

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