Nitrogen-Containing Bisphosphonates Downregulate Cathepsin K and Upregulate Annexin V in Osteoclasts Cultured In Vitro

Figure 3

Red TRAP-stained bone marrow-derived cells were imaged with the Plus 2.0 software (Motic Images Plus 2.0, Canada) and an inverted microscope (Motic AE 2000, USA). Three sites were randomly selected and evaluated in each well of a 24-well plate. Positive TRAP staining was quantified as >3 nuclei per cell (220 ± 32.56) using the ImageJ software (ImageJ/Fiji 1.53a, NIH, USA).