Prolactin and Male Fertility: The Long and Short Feedback Regulation
Figure 2
Age-related changes in serum concentrations of
prolactin (dotted line), release of prolactin by whole pituitary (free release; dashed line),
hypothalamic prolactin-releasing activity (solid line), and hypothalamic prolactin-inhibiting activity (dotted-dashed line) in male rats.
Values are mean ± SEM from
Values are mean ± SEM from
two separate experiments and a minimum number of 14 animals per pooled group. * compared with preceding group; † compared with hypothalamic
prolactin-releasing activity at the same age (Student’s t-test). The inset shows the results for days 7 and 14 on an expanded
scale. The pituitary constructs were dissected
out of 7 to 77 day-old rats and incubated in DMEM for 4 hours at 37°C.
Prolactin was estimated by RIA in the spent medium [35].
