Prolactin and Male Fertility: The Long and Short Feedback Regulation
Figure 4
Intrapituitary contents of PRL after incubation of
PHC (left-hand panel)
and PI (right-hand panel) from normal rats without (stippled bars) or with (hatched bars) testosterone (0.3 mM) or from castrated rats
incubated without (open bars) or with (solid bars) testosterone (0.3 mM) for 72 hours. Values represent mean ± SE derived from 10 pituitaries. p, q, r, denote significant
differences in groups incubated with or without testosterone, normal controls versus
castrated controls and between normal incubated with testosterone versus castrated
incubated with testosterone, respectively. *. C: Incubation without
testosterone; T: Incubation with testosterone. Pituitary prolactin content was
estimated by RIA in PBS homogenates of pituitary constructs incubated with or
without 0.3 mM testosterone for 72 hours and a challenge dose of LHRH at 37°C for
4 hours [54].
