Prolactin and Male Fertility: The Long and Short Feedback Regulation
Figure 9
(a) Spermatozoa
taken from untreated rats that have not picked up the nuclear chromatin stain
viewed under fluorescent microscope at 100 X magnification at 500–610 nm
excitation/fluorescence emission wavelength. (b) Spermatozoa of untreated rats that have not picked up the stain viewed
under phase contrast objective at 100 X magnification. (c)-(d) Spermatozoa taken from the caput epididymides of fluphenazine-treated
rats that have picked up the intense yellow CMA3 nuclear chromatin stain in the
GC-rich regions occupied normally by protamine. Spermatozoa were fixed in
Carnoy’s fixative and stained with CMA3 dye in McIlvaine’s buffer. Staining was
viewed under fluorescent microscope at 100 X magnification at 500–610 nm
excitation/fluorescence emission wavelength.