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International Journal of Endocrinology
Volume 2009, Article ID 875167, 6 pages
http://dx.doi.org/10.1155/2009/875167
Research Article

Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells

Service de Gastroentréologie, Département de Médecine, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4

Received 14 May 2008; Revised 3 September 2008; Accepted 29 September 2008

Academic Editor: Andre Marette

Copyright © 2009 Karim El-Kouhen and Jean Morisset. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

With the exclusive presence of the pancreatic CCK-2 receptors on the pancreatic delta cells of six different species, this study was undertaken to determine the role of cholecystokinin and gastrin on growth of these somatostatin (SS) cells. For this study, the SS-RIN-14B cells were used in culture and their growth was evaluated by cell counting. Results. To our surprise, we established by Western blot that these RIN cells possess the two CCK receptor subtypes, CCK-1 and CCK-2. Occupation of the CCK-1 receptors by caerulein, a CCK analog, led to inhibition of cell proliferation, an effect prevented by a specific CCK-1 receptor antagonist. Occupation of the CCK-2 receptors by the gastrin agonist pentagastrin had no effect on cell growth. Proliferation was not affected by SS released from these cells but was inhibited by exogenous SS. Conclusions. Growth of the SS-RIN-14B cells can be negatively affected by occupation of their CCK-1 receptors and by exogenous somatostatin.