Thyroid Hormone Increases TGF-1 in Cardiomyocytes Cultures Independently of Angiotensin II Type 1 and Type 2 Receptors
Figure 1
TGF-β1 protein expression in cardiac fibroblasts (a) and cardiomyocytes cultures (b), evaluated by Western Blot. TGF-β1 gene expression in cardiac fibroblasts (c) and cardiomyocytes cultures (d), evaluated by RT-PCR. The cells were treated for 24 hours with serum-free medium containing 10 nM of () or only serum-free medium in control cells. -actinin was used for normalization of TGF-β1 protein expression and -actin was used for normalization of TGF-β1 gene expression. The values are expressed in percentage. versus control ().