Vitamin DView this Special Issue
Research Article | Open Access
Lars Rejnmark, Peter Vestergaard, Lene Heickendorff, Leif Mosekilde, "Simvastatin Does Not Affect Vitamin D Status, but Low Vitamin D Levels Are Associated with Dyslipidemia: Results from a Randomised, Controlled Trial", International Journal of Endocrinology, vol. 2010, Article ID 957174, 6 pages, 2010. https://doi.org/10.1155/2010/957174
Simvastatin Does Not Affect Vitamin D Status, but Low Vitamin D Levels Are Associated with Dyslipidemia: Results from a Randomised, Controlled Trial
Objectives. Statin drugs act as inhibitors of the 3-hydroxy-3methylglutaryl coenzyme A (HMG-CoA) reductase enzyme early in the mevalonate pathway, thereby reducing the endogenous cholesterol synthesis. In recent studies, it has been suggested from epidemiological data that statins also may improve vitamin D status, as measured by increased plasma 25-hydroxyvitamin D (25OHD) levels. We now report the results from a randomised controlled trial on effects of simvastatin on plasma 25OHD levels. Design and Methods. We randomised 82 healthy postmenopausal women to one year of treatment with either simvastatin 40 mg/d or placebo and performed measurement at baseline and after 26 and 52 weeks of treatment. The study was completed by 77 subjects. Results. Compared with placebo, plasma levels of cholesterol and low-density lipoproteins decreased in response to treatment with simvastatin, but our study showed no effect of simvastatin on vitamin D status. However, plasma levels of triglycerides were inversely associated with tertiles of plasma 25OHD levels and changes in plasma triglycerides levels correlated inversely with seasonal changes in vitamin D status. Conclusion. Our data do not support a pharmacological effect of statins on vitamin D status, but do suggest that vitamin D may influence plasma lipid profile and thus be of importance to cardiovascular health.
During recent years, treatment with statins has been suggested to cause positive effects on bone. Although, discrepant results have been reported in clinical studies on effects of statins on bone mineral density (BMD) and bone turnover [1–5], several epidemiological studies have shown that treatment with statins is associated with a reduced risk of fracture [6–10]. Several mechanisms of action of statins on bone have been suggested. Statins may exert a bone anabolic action due to an increased osteoblastic synthesis of bone morphogenetic protein 2 (BMP-2), a growth factor that causes osteoblastic proliferation , as well as antiresorptive effects similar to nitrogen-containing bisphosphonates (amino-BP) . Moreover, an effect of statins on vitamin D metabolism has been suggested as an additional mechanism of action by which statins may exert pleiotropic effects. In several [13–16] but not all [17–19] studies, treatment with statins has been associated with an improved vitamin D status. An emerging amount of evidence suggests that an impaired vitamin D status increases the risk of different types of cancers and chronic disorders, including cardiovascular diseases [20, 21]. If statins improve vitamin D status this could be a plausible explanation for the findings of not only a decreased fracture risk, but also a decreased risk of malignant diseases in users of statin drugs . Thus, in a randomised controlled design we studied effects of one year of simvastatin treatment on vitamin D status in a group of healthy postmenopausal women.
2. Subjects and Methods
In year 2000, we initiated a study on effects of statin treatment on bone. The design and major results of the study has previously been detailed . In brief, in a double-blinded design we randomised 82 healthy Caucasian women to one year of treatment with either simvastatin 40 mg/d or placebo. In addition, all studied subjects received a daily supplement with 400 mg of elementary calcium, but no vitamin D supplementations. Calcium and simvastatin tablets were obtained commercially.
We recruited studied subjects, through invitations by letter, from a random sample of the general background population. We only included women who were more than 12 months postmenopause and below 76 years of age. In addition, we required studied subjects to be healthy as assessed by a standard biochemical screening program and to have osteopenia at the lumbar spine or total hip, that is, a BMD less than 1 standard deviation (SD) below the mean of peak bone mass (T-score 1). We excluded women with diseases or use of drugs known to affect calcium homeostasis and bone metabolism, including impaired renal (plasma creatinine 120 mol/L) or hepatic (plasma alanine aminotransferase 80 U/L) function, and alcohol abuse of more than 14 units a week within the last 2 years. None of studied subjects had known hyperlipidemia prior to study start. As a safety measure, we excluded subjects with low plasma cholesterol levels (total cholesterol 4.0 mmol/L and/or LDL 2.5 mmol/L).
The study was carried out in accordance with the Declaration of Helsinki II. It was approved by the regional Ethical Committee (Aarhus County # 2000/0223) and the Danish National Board of Health. Each individual gave verbal and written informed consent prior to the study. The Good Clinical Practice (GCP) Unit at the University Hospital of Aarhus, Denmark, monitored the study.
During trial, blood samples were drawn between 7.00 a.m. and 10.30 a.m. after an overnight fast. At time of study, we analysed plasma levels of calcium, creatinine, albumin, total cholesterol (TC), LDL cholesterol (LDL), HDL cholesterol (HDL), and triglyceride (TG) by standard laboratory methods. We now report measurements of plasma 25-hydroxyvitamin D (P-25OHD) levels in samples collected at baseline and after 6 and 12 months of treatment. Since collected, all samples have been stored at C. We analysed P-25OHD levels using an isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method adapted from Maunsell et al. . Mean coefficients of variation for 25OHD3 were 6.4% and 9.1% at levels of 66.5 and 21.1 nmol/L and for 25OHD2 the CV values were 8.8% and 9.4% at levels of 41.2 and 25.3 nmol/L.
We assessed differences between study groups using Chi-square tests for categorical variables and a two-sample t -test or Mann-Whitney U-test for continuous variables, as appropriate. For this analysis, we had stored samples for 77 study subjects who completed the one year of treatment (38 in the placebo group and 39 in the statin group). Accordingly, data are presented as a per-protocol analysis. Serial changes were studied using analysis of variance (ANOVA) for repeated measurements (RM-ANOVA), with treatment group as the independent variable (effect of time by group). Assumptions for repeated measures ANOVA were checked by Mauchly’s test of sphericity, and accordingly adjustment in the degrees of freedom was made (Huynh-Feldt epsilon). In case of a significant between-groups difference by repeated measures ANOVA, differences between groups were analysed at each time point of measurements by a posterior analysis using a two-sample test. We assessed association between studied parameters using correlations and multiple regression analyses. All results are given as mean ± standard error of the mean (SEM) unless otherwise stated. Statistical analysis was performed using Statistical Package for Social Sciences (SPSSs 14.0) for Windows.
Women included in the study had a median age of 64 years (range 53 to 74 years). Baseline characteristics including P-25OHD levels did not differ statistically between groups, except that women randomised to placebo by change were heavier than subjects randomised to simvastatin treatment (Table 1). Only three of our studied subjects had vitamin D deficiency defined as a P-25OHD level 25 nmol/L, whereas 11 (14%) had vitamin D insufficiency (P-25OHD 50 nmol/L). One third of our studied subjects had a P-25OHD level 80 nmol/L (Table 1). As previously reported, 52 weeks of treatment with simvastatin caused, compared with placebo, a significant decrease in plasma levels of cholesterol (27%, 95% confidence interval (CI), 22% to 32%, ) and LDL-cholesterol (45%, 95% CI, 38% to 52%, ), whereas plasma levels of HDL-cholesterol and triglycerides did not change significantly in response to treatment . Similarly, statin treatment did not affect plasma levels of parathyroid hormone or biochemical markers of bone turnover, including plasma levels of C-terminal telopeptide of type I (CTX), collagen osteocalcin, and bone specific alkaline phosphatase .
5.1. Effects of Simvastatin Treatment on Plasma 25OHD Levels
Treatment with simvastatin did not affect P-25OHD levels compared with placebo ( by RM-ANOVA, Figure 1). P-25OHD levels did not change significantly between baseline and week 52 within the group of women treated with simvastatin ( by paired sample test) or placebo . As shown in Figure 1, P-25OHD levels were higher in both study groups at week 26 compared with values at baseline and week 52, which is attributable to seasonal variations. Samples at baseline and week 52 were collected during wintertime (October to April), whereas samples at week 26 were collected during summertime (April to October).
5.2. Associations between Vitamin D- and Cholesterol-Status
In order to assess whether P-25OHD levels influence cholesterol status, we analysed baseline indices of cholesterol status, as measured by plasma levels of TC, LDL, HDL, or TG by tertiles of P-25OHD levels (Table 2). Women in the highest tertile of P-25OHD levels (80 nmol/L) had significantly lower plasma levels of TG than those in the lowest P-25OHD tertile, whereas plasma HDL levels increased borderline significantly by P-25OHD tertiles (Table 2). Dividing studied subjects into groups pf vitamin D status according to the often used cutoff limits for vitamin D status, that is, P-25OHD 50, between 50–80, and 80 nmol/L showed very similar results with significantly lower TG levels ( mmol/L) in vitamin D replete women (P-25OHD 80 nmol/L) than in women with vitamin D insufficiency (P-25OHD 50 nmol/L: TG mmol/L, ). Moreover, on averages, P-25OHD levels increased from nmol/L at wintertime (baseline) to nmol/L at summertime (week 26). Concomitantly, plasma TG levels decreased from mmol/L to mmol/L. In a linear regression analysis, adjusted for treatment allocation, the seasonal changes in P-25OHD levels were significantly associated with the concomitant changes in plasma TG levels (, , ). Similarly, the decrease in P-25OHD levels between week 26 and 52 (from summer- to winter-time) correlated significantly with the concomitant changes in plasma TG levels (, , ). Further adjustments for BMI did not change the results.
| compared with lowest tertile by post-hoc test.|
In a randomised, controlled study, we found no effects on plasma 25OHD levels of one year of treatment with simvastatin 40 mg/d compared with placebo. However, our analysis showed an effect of vitamin D status on plasma levels of TG, a finding that may contribute to our understanding of the potential positive effects of vitamin D on cardiovascular health.
For more than two decades, statins have been used to reduce cholesterol levels in patients with cardiovascular diseases. They act as HMG-CoA reductase inhibitors, thereby reducing the endogenous cholesterol synthesis. When statins were introduced, it was a matter of concern whether inhibition of the cholesterol biosynthetic pathway may affect other metabolic processes which are dependent on intermediates from this pathway. Especially, concerns have been paid to the reduced tissue concentrations of 7-dehydrocholesterol (7-DHC) in response to treatment with statins. As 7-DHC is the precursor for endogenous skin synthesis of cholecalciferol, reduced levels of 7-DHC may impair vitamin D status. However, in a study including 17 men and women on treatment with pravastatin and 14 hypercholesterolemic age and gender matched controls, vitamin D levels increased in a similar manner in both groups in response to exposure of the skin surface to type B ultraviolet (UV-B) radiation, indicating no harmful effects of pravastatin on the endogenous vitamin D synthesis . On the contrary, in several papers statin therapy has been suggested to improve vitamin D status. In a group of 83 Spanish men and women with acute coronary syndrome in whom treatment with atorvastatin was initiated, vitamin D status as measured by P-25OHD levels improved. Thus, during one year of observation P-25OHD levels increased from nmol/L at baseline to nmol/L after 12 months, which, according to the investigators, was attributable to treatment with atorvastatin . Similar results have been reported by other investigators [15, 16], including a cross-sectional analysis showing increased P-25OHD levels in patients on treatment with statins . In contrast to these findings from observational studies, the results from our randomised controlled study showed no effect of simvastatin treatment on P-25OHD levels. Although we cannot exclude differential effects on vitamin D status of different types of statin drugs (e.g., atorvastatin versus simvastatin), we find it most likely that the findings from the uncontrolled studies are due to unmeasured changes in indices affecting P-25OHD levels. In general, P-25OHD levels are determined by intake from food items rich in vitamin D (especially fatty fish), use of vitamin D supplements, and sun exposure. To the best of our knowledge, none of these indices was controlled for in the hitherto published uncontrolled studies on possible effects of statins on vitamin D status. Most likely, the findings from these studies are due to the coincidence of changes in life-style habits in relation to administration of statin drugs.
Vitamin D has, however, independently of treatment with statins, been implicated in cardiovascular health and atherogenesis . Results from a randomised controlled study showed a decreased blood pressure in response to UVB exposure , and P-25OHD levels have been shown to correlate inversely with risk of atherosclerosis in several cohort studies [21, 27, 28]. However, other investigators have reported either no effects or even detrimental effects on plasma lipid levels in response to administration of vitamin D [29–32]. The vitamin D receptor is expressed by both cardiac myocytes, endothelial-, and smooth vascular muscle-cells and these cells also express the 1 -hydroxylase enzyme, that is, they may locally activate vitamin D from its circulation precursor (25OHD) to its active metabolite (1,25-dihydroxyvitamin D) [33, 34]. Several mechanisms of action have been implicated in the potential antiatherosclerotic effects of vitamin D, including a downregulation of the renin-angiotensin system, and a reduced expression of mRNA and protein levels of plasminogen activator inhibitor-1 (PAI-1) and thrombospondin-1 (THBS1) which are known to be involved in the development of atherosclerosis [35, 36]. In addition, our data points toward a further mechanism by which vitamin D may protect against cardiovascular diseases, that is, through decreased TG levels . A possible mechanism of action by which vitamin D lowers TG levels is through an increased activity of the lipoprotein lipase, which has been shown to be regulated by vitamin D in adipocytes . Our data on effects of vitamin D on TG levels are limited by the cross-sectional design of the analysis and included only women. Further randomised controlled studies on effects of vitamin D on plasma lipid profile are warranted, including studies in men.
In conclusion, results from our randomised, controlled trial do not support an effect of statin treatment on vitamin D status as determined by measurement of plasma 25OHD levels. However, P-25OHD levels may improve the plasma lipid profile and thereby risk of cardiovascular disease.
- L. Rejnmark, N. H. Buus, P. Vestergaard et al., “Effects of simvastatin on bone turnover and BMD: a 1-year randomized controlled trial in postmenopausal osteopenic women,” Journal of Bone and Mineral Research, vol. 19, no. 5, pp. 737–744, 2004.
- H. G. Bone, D. P. Kiel, R. S. Lindsay et al., “Effects of atorvastatin on bone in postmenopausal women with dyslipidemia: a double-blind, placebo-controlled, dose-ranging trial,” Journal of Clinical Endocrinology and Metabolism, vol. 92, no. 12, pp. 4671–4677, 2007.
- L. Rejnmark, N. H. Buus, P. Vestergaard, F. Andreasen, M. L. Larsen, and L. Mosekilde, “Statins decrease bone turnover in postmenopausal women: a cross-sectional study,” European Journal of Clinical Investigation, vol. 32, no. 8, pp. 581–589, 2002.
- C. J. Edwards, D. J. Hart, and T. D. Spector, “Oral statins and increased bone-mineral density in postmenopausal women,” The Lancet, vol. 355, no. 9222, pp. 2218–2219, 2000.
- G. Lupattelli, A. M. Scarponi, G. Vaudo et al., “Simvastatin increases bone mineral density in hypercholesterolemic postmenopausal women,” Metabolism, vol. 53, no. 6, pp. 744–748, 2004.
- L. Rejnmark, M. L. Olsen, S. P. Johnsen, P. Vestergaard, H. Sorensen, and L. Mosekilde, “Hip fracture risk in statin users: a population-based Danish case-control study,” Osteoporosis International, vol. 15, no. 6, pp. 452–458, 2004.
- L. Rejnmark, P. Vestergaard, and L. Mosekilde, “Statin but not non-statin lipid-lowering drugs decrease fracture risk: a nation-wide case-control study,” Calcified Tissue International, vol. 79, no. 1, pp. 27–36, 2006.
- T.-P. van Staa, S. Wegman, F. de Vries, B. Leufkens, and C. Cooper, “Use of statins and risk of fractures,” Journal of the American Medical Association, vol. 285, no. 14, pp. 1850–1855, 2001.
- D. C. Bauer, G. R. Mundy, S. A. Jamal et al., “Use of statins and fracture: results of 4 prospective studies and cumulative meta-analysis of observational studies and controlled trials,” Archives of Internal Medicine, vol. 164, no. 2, pp. 146–152, 2004.
- C. Hatzigeorgiou and J. L. Jackson, “Hydroxymethylglutaryl-coenzyme—a reductase inhibitors and osteoporosis: a meta-analysis,” Osteoporosis International, vol. 16, no. 8, pp. 990–998, 2005.
- G. Mundy, R. Garrett, S. Harris et al., “Stimulation of bone formation in vitro and in rodents by statins,” Science, vol. 286, no. 5446, pp. 1946–1949, 1999.
- A. Staal, J. C. Frith, M. H. French et al., “The ability of statins to inhibit bone resorption is directly related to their inhibitory effect on HMG-CoA reductase activity,” Journal of Bone and Mineral Research, vol. 18, no. 1, pp. 88–96, 2003.
- J. L. Perez-Castrillon, L. Abad, G. Vega et al., “Effect of atorvastatin on bone mineral density in patients with acute coronary syndrome,” European Review for Medical and Pharmacological Sciences, vol. 12, no. 2, pp. 83–88, 2008.
- J. F. Aloia, M. Li-Ng, and S. Pollack, “Statins and vitamin D,” American Journal of Cardiology, vol. 100, no. 8, p. 1329, 2007.
- H. Wilczek, J. Sobra, V. Justova et al., “Iatropathogenic effect of MEVACOR on vitamin D metabolism,” Casopis Lekaru Ceskych, vol. 128, no. 40, pp. 1254–1256, 1989.
- H. Wilczek, J. Sobra, R. Ceska et al., “Monitoring plasma levels of vitamin D metabolites during simvastatin (Zocor) treatment in patients with familial hypercholesterolaemia,” Casopis Lekaru Ceskych, vol. 133, no. 23, pp. 727–729, 1994.
- M. Montagnani, F. Lore, G. di Cairano et al., “Effects of pravastatin treatment on vitamin D metabolites,” Clinical Therapeutics, vol. 16, no. 5, pp. 824–829, 1994.
- F. Ismail, C. N. Corder, S. Epstein, G. Barbi, and S. Thomas, “Effects of pravastatin and cholestyramine on circulating levels of parathyroid hormone and vitamin D metabolites,” Clinical Therapeutics, vol. 12, no. 5, pp. 427–430, 1990.
- A. S. Dobs, M. A. Levine, and S. Margolis, “Effects of pravastatin, a new HMG-CoA reductase inhibitor, on vitamin D synthesis in man,” Metabolism, vol. 40, no. 5, pp. 524–528, 1991.
- M. F. Holick, “Vitamin D deficiency,” The New England Journal of Medicine, vol. 357, no. 3, pp. 266–281, 2007.
- E. Giovannucci, Y. Liu, B. W. Hollis, and E. B. Rimm, “25-hydroxyvitamin D and risk of myocardial infarction in men: a prospective study,” Archives of Internal Medicine, vol. 168, no. 11, pp. 1174–1180, 2008.
- W. R. Farwell, R. E. Scranton, E. V. Lawler et al., “The association between statins and cancer incidence in a veterans population,” Journal of the National Cancer Institute, vol. 100, no. 2, pp. 134–139, 2008.
- Z. Maunsell, D. J. Wright, and S. J. Rainbow, “Routine isotope-dilution liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of the 25-hydroxy metabolites of vitamins D2 and D3,” Clinical Chemistry, vol. 51, no. 9, pp. 1683–1690, 2005.
- J. L. Perez-Castrillon, G. Vega, L. Abad et al., “Effects of atorvastatin on vitamin D levels in patients with acute ischemic heart disease,” The American Journal of Cardiology, vol. 99, no. 7, pp. 903–905, 2007.
- M. L. Melamed, E. D. Michos, W. Post, and B. Astor, “25-hydroxyvitamin D levels and the risk of mortality in the general population,” Archives of Internal Medicine, vol. 168, no. 15, pp. 1629–1637, 2008.
- R. Krause, M. Buhring, W. Hopfenmuller, M. F. Holick, and A. M. Sharma, “Ultraviolet B and blood pressure,” The Lancet, vol. 352, no. 9129, pp. 709–710, 1998.
- R. Scragg, R. Jackson, I. M. Holdaway, T. Lim, and R. Beaglehole, “Myocardial infarction is inversely associated with plasma 25-hydroxyvitamin D3 levels: a community-based study,” International Journal of Epidemiology, vol. 19, no. 3, pp. 559–563, 1990.
- D. Martins, M. Wolf, D. Pan et al., “Prevalence of cardiovascular risk factors and the serum levels of 25-hydroxyvitamin D in the United States: data from the Third National Health and Nutrition Examination Survey,” Archives of Internal Medicine, vol. 167, no. 11, pp. 1159–1165, 2007.
- L. A. Carlson, H. Derblom, and A. Lanner, “Effect of different doses of vitamin D on serum cholesterol and triglyceride levels in healthy men,” Atherosclerosis, vol. 12, no. 2, pp. 313–317, 1970.
- M. H. Gannage-Yared, M. Azoury, I. Mansour, R. Baddoura, G. Halaby, and R. Naaman, “Effects of a short-term calcium and vitamin D treatment on serum cytokines, bone markers, insulin and lipid concentrations in healthy post-menopausal women,” Journal of Endocrinological Investigation, vol. 26, no. 8, pp. 748–753, 2003.
- M. Tuppurainen, A.-M. Heikkinen, I. Penttilä, and S. Saarikoski, “Does vitamin D3 have negative effects on serum levels of lipids? A follow-up study with a sequential combination of estradiol valerate and cyproterone acetate and/or vitamin D3,” Maturitas, vol. 22, no. 1, pp. 55–61, 1995.
- R. H. K. Mak, “1,25-dihydroxyvitamin D3 corrects insulin and lipid abnormalities in uremia,” Kidney International, vol. 53, no. 5, pp. 1353–1357, 1998.
- J. Merke, P. Milde, S. Lewicka et al., “Identification and regulation of 1,25-dihydroxyvitamin D3 receptor activity and biosynthesis of 1,25-dihydroxyvitamin D3. Studies in cultured bovine aortic endothelial cells and human dermal capillaries,” Journal of Clinical Investigation, vol. 83, no. 6, pp. 1903–1915, 1989.
- T. D. O'Connell, J. E. Berry, A. K. Jarvis, M. J. Somerman, and R. U. Simpson, “1,25-dihydroxyvitamin D3 regulation of cardiac myocyte proliferation and hypertrophy,” American Journal of Physiology, vol. 272, no. 4, pp. H1751–H1758, 1997.
- J. R. Wu-Wong, M. Nakane, and J. Ma, “Vitamin D analogs modulate the expression of plasminogen activator inhibitor-1, thrombospondin-1 and thrombomodulin in human aortic smooth muscle cells,” Journal of Vascular Research, vol. 44, no. 1, pp. 11–18, 2007.
- Y. C. Li, G. Qiao, M. Uskokovic, W. Xiang, W. Zheng, and J. Kong, “Vitamin D: a negative endocrine regulator of the renin-angiotensin system and blood pressure,” Journal of Steroid Biochemistry and Molecular Biology, vol. 89-90, no. 1–5, pp. 387–392, 2004.
- N.-A. Le and M. F. Walter, “The role of hypertriglyceridemia in atherosclerosis,” Current Atherosclerosis Reports, vol. 9, no. 2, pp. 110–115, 2007.
- U. Querfeld, M. M. Hoffmann, G. Klaus et al., “Antagonistic effects of vitamin D and parathyroid hormone on lipoprotein lipase in cultured adipocytes,” Journal of the American Society of Nephrology, vol. 10, no. 10, pp. 2158–2164, 1999.
Copyright © 2010 Lars Rejnmark et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.