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International Journal of Endocrinology
Volume 2011, Article ID 707928, 12 pages
Research Article

Liver GlucokinaseA456V Induces Potent Hypoglycemia without Dyslipidemia through a Paradoxical Induction of the Catalytic Subunit of Glucose-6-Phosphatase

Biophysics Unit, Department of Physiological Sciences II, IDIBELL-University of Barcelona, Campus de Bellvitge, 08907 L'Hospitalet de Llobregat, Spain

Received 4 August 2011; Accepted 9 September 2011

Academic Editor: Maria L. Dufau

Copyright © 2011 Anna Vidal-Alabró et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure 1. pGK and pGKA456V analysis in vitro. (A) Comparision between glucokinase activity at different glucose concentrations of hepatoma cells Huh-7 which have been transfected with pGK (continuous line) and pGKA456V (discontinuous line), respectively. (B) Activity homogenates were also used to evaluate through Western Blot the expression levels of pGK and pGKA456V. “pC” lane corresponds to an homogenate of Huh-7 cells transfected with a pEGFP plasmid and “+” is the positive control and consists of a fed mouse liver homogenate.

  1. Supplementary Material