A Cell Model for Conditional Profiling of Androgen-Receptor-Interacting Proteins

Figure 4

AR interacting proteins SMARCC1 and NR3C1 are differentially expressed in proliferating versus nonproliferating P17 cells. Western blot analysis of known AR coregulators identified by mass spectrometry only in AR FLAG copurifications from proliferating P17 cells (SMARCC1) and of a known coregulator identified by mass spectrometry only in AR FLAG copurifications from nonproliferating P17 cells (NR3C1). SRC1 was not identified at all, neither in purifications from proliferating nor nonproliferating P17 cells. AR and ACTB were identified in both FLAG copurifications from P17 cells by mass spectrometry. Compared to the other identified known AR interacting proteins NR3C1, SMARCC1, and ACTB, a smaller portion of the total SRC1 present in the nuclear extract (P17 Input) interacts with AR (P17 TEV). M is the size estimate by a prestained protein ladder in kilo Daltons (kDa).