Distribution of Urocortins and Corticotropin-Releasing Factor Receptors in the Cardiovascular System

Figure 2

RT-PCR of urocortin 3 (Ucn III) mRNA in brain, pituitary, heart, and kidneys. Cortex: cerebral cortex; Hypot: hypothalamus; Pitui: pituitary; RA: right atrium; RV: right ventricle; LA: left atrium; LV: left ventricle; Kid 1 and Kid 2: kidneys. RT(−) indicates negative controls (samples without reverse-transcriptase treatment). A 469 base-pair DNA fragment corresponding to 5/473 of the human urocortin 3 gene (GeneBank accession number AF361943) was amplified by RT-PCR (reproduced from [30] with kind permission from the Endocrine Society. Copyright 2004, The Endocrine Society).