Synergistic Effect of Hyperglycemia and
Suppression on Adult Mouse Islet Beta Cell Replication
Figure 1
Effect of p27kip1 silencing on the expression of islet cell cycle proteins. Total islet cell protein contents of targeting islets were prepared right after isolation (0 hr) and at 54 and 96 hours (hrs) after viral infection; separated on a 12% SDS gel and electrophoretically transferred onto nitrocellulose membranes. After checking transfer efficiency, the membrane was blocked and incubated overnight in buffer containing 2 μg/mL each of rabbit antibodies against mouse p27kip1, cyclin D1, β-actin, cyclin B1, and FoxM1; incubated with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G antibody. The bound antibodies were detected by using chemiluminescent kit and film development. The molecular weight is 27 kD for p27, 36 kD for cyclin D1, 43 kD for β-actin, 62 kD for cyclin B1, and 84 kD for FoxM1.
