Synergistic Effect of Hyperglycemia and
Suppression on Adult Mouse Islet Beta Cell Replication
Figure 2
Short-term ex vivo effect of p27Kip1 silencing on islet cell proliferation in diabetic syngeneic recipients. Batches of 50 of targeting (a) or non-targeting (b) transduced islets were implanted in the subcapsular space of the left kidney of B6 mice with streptozotocin-induced diabetes, and grafts were removed 6 days later for immunohistochemistry by using the anti-Ki67 antibody. The deep-colored oval structures represent positively stained nuclei. The black scale bar indicates a length of 10 micrometers.
