Synergistic Effect of Hyperglycemia and p27^kip1 Suppression on Adult Mouse Islet Beta Cell Replication

Figure 4

Curves of cumulative diabetes mellitus cure rate for syngeneic islet transplantation. Each streptozotocin-induced diabetic mouse was transplanted with 200 mouse islets transduced with either shRNAs to p27^kip1 or the non-target shRNA control implanted underneath the left renal capsule at day 0. Cure of diabetes was defined as the stable restoration of normoglycemia (<200 mg/dL glucose). Percent cure 50 (%) stands for half number of the transplanted diabetic mice becoming normoglycemia. The beneficial effect of p27^kip1 silencing on the function of islet grafts was reflected by the high cumulative cure rate of diabetes. The difference between two groups was analyzed with the log rank test. The value of the comparison for targeting versus non-targeting was 𝑃<0.05.