Research Article
Synergistic Effect of Hyperglycemia and Suppression on Adult Mouse Islet Beta Cell Replication
Table 2
The effect of p27kip1 silencing on islet glucose-stimulation insulin secretion in vitro. After 24 h of transduction and at 3 and 7 days of culturing, batches of 30 islets per well were sequentially exposed to different concentrations of glucose. Islets were incubated in 100 mg/dL glucose for 60 min. After the medium was collected, the same batch of islets was washed and then stimulated with 360 mg/dL of glucose for 60 min. The net difference of insulin secretion from the glucose stimulation test (GSIS; ng/islet × 60 min) was calculated as the difference of the insulin content between the medium containing 360 mg/dL and medium containing 100 mg/dL glucose. The stimulation index (SI) was considered as the ratio of insulin content of the medium containing 360 mg/dL of glucose over that of the medium containing 100 mg/dL of glucose for the same batch of islets.
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