Synergistic Effect of Hyperglycemia and p27^kip1 Suppression on Adult Mouse Islet Beta Cell Replication

Table 3

Effect of p27^Kip1 silencing on the period of temporary hyperglycaemia and insulin contents of islet graft and pancreatic remnant. Batches of 200 isogeneic islets with targeting and non-targeting (control) transduction were implanted in the subcapsular space of the left kidney of B6 mice with streptozotocin-induced diabetes. After transplantation, blood glucose was measured, and the duration of temporary hyperglycemia was the period between transplantation and the stable restoration of normoglycemia (<200 mg/dL). The graft-bearing kidneys were removed 12 weeks after transplantation for the measurement of insulin contents. The pancreatic remnants were removed 2 weeks after nephrectomy for measuring the insulin content. ^a𝑃<0.05 when the indicated means were analyzed using unpaired Student’s t-test.

No. of Islets	Lentivirus	N	Period of temporary hyperglycaemia (days)	GIC (μg)	PIC (μg)
200	Targeting	13	16.5±2.9^a	1.75±0.18	0.22±0.07
200	Control	14	25.9±3.5^a	1.54±0.17	0.22±0.05