Exendin-4 Protects MIN6 Cells from t-BHP-Induced Apoptosis via IRE1-JNK-Caspase-3 Signaling

Figure 1

Exendin-4 inhibits t-BHP-induced β-cell apoptosis. MIN6 cells were preincubated with exendin-4 (100 nM) or with SP600125 (10 nM) for 18 h and then exposed to t-BHP (25 μM) for 1 h. The rate of apoptosis was determined by Hoechst/PI staining (A) and flow cytometry (B). The cells containing bright blue particles (apoptosis-positive cells) were visualized under a light microscope (A). (a) Control; (b) t-BHP alone; (c) exendin-4 (Ex-4) alone; (d) Ex-4 + t-BHP; (e) SP600125 + t-BHP; (f) SP600125; (g) quantification of apoptotic cells. Values are expressed as the mean ± SD (𝑛=3). ^∗∗∗𝑃<0.001compared with the control group; ^###𝑃<0.001versus t-BHP alone.