Exendin-4 Protects MIN6 Cells from t-BHP-Induced Apoptosis via IRE1-JNK-Caspase-3 Signaling

Figure 2

Exendin-4 inhibits caspase-3 activity in t-BHP-treated MIN6 cells. MIN6 cells were preincubated with exendin-4 (100 nM) or with SP600125 (10 nM) for 18 h and then exposed to t-BHP (25 μM) for 1 h. Caspase-3 activity was determined using a caspase-3 activity assay (a) and western blotting (b), as described in Section 2. The histogram shows the quantification of the protein data. Levels of active caspase-3 protein were normalized to caspase-3 levels and expressed as the relative fold change compared to the control samples. Values are expressed as the mean ± SD (𝑛=3). ^∗∗∗𝑃<0.001 versus control group; ^###𝑃<0.001 versus the t-BHP group.

(a)

(b)