Metabolic Context Regulates Distinct Hypothalamic Transcriptional Responses to Antiaging Interventions
Figure 4
Wheel running influences hypothalamic gene expression and pathway activation in C57Bl/6 mice. (a) Z-ratios for gene transcripts up- or downregulated following voluntary wheel running in C57Bl/6 mice. Fkbp5, FK506 binding protein 5; Vps72, vacuolar protein sorting 72; Sh3gl1, SH3-domain GRB2-like 1; Lcn2, lipocalin 2; Hcrt, hypocretin; Trim72, tripartite motif-containing 72. (b), Pathways responsive to running in the hypothalamus of C57Bl/6 mice. Abbreviations: Lep, leptin; Htr1b, 5-hydroxytryptamine receptor 1B; Tsn, translin; Sfrp1, secreted frizzled-related protein 1; Slc30a3, solute carrier family 30, member 3; Slit1, slit homolog 1; Pou3f3, POU domain, class 3, transcription factor 3; Chrd, chordin; Nkx2-1, NK2 homeobox 1; Pgr, progesterone receptor. (c), PCR validation of running-induced transcriptional alterations in the hypothalamus of wild-type mice. (d) Leptin mRNA was detected in the hypothalamus samples using microarray, PCR, and in situ hybridization techniques. White adipose tissue (WAT) expresses leptin mRNA at higher levels than hypothalamus (Hyp) by PCR; ob/ob mice lack leptin bands at 155 bp in the hypothalamus following restriction enzyme digest, while wild-type (wt) controls express leptin mRNA. As shown by the microarray, running reduced endogenous leptin mRNA expression in the hypothalamus. In situ hybridization techniques were also used to detect endogenous leptin mRNA expression in the hypothalamus of sedentary wild-type mice.
