Metabolic Context Regulates Distinct Hypothalamic Transcriptional Responses to Antiaging Interventions

Figure 7

CR activates a diverse set of transcripts and pathways in db/db mice. (a) Z-ratios for genes that were up- or downregulated following CR in db/db mice. Fpr2, formyl peptide receptor 2; Pmch, pro-melanin-concentrating hormone; Oxt, oxytocin; Kif2a, kinesin family member 2A; H2afj, H2A histone family, member J; Scn1a, sodium channel, voltage-gated, type I, alpha. (b) Pathway analysis of genes responsive to restricted feeding in leptin receptor-deficient mice. Abbreviations: Il6st, interleukin 6 signal transducer; Ccnd2, cyclin D2; Prkaa2, protein kinase, AMP activated, alpha 2 catalytic subunit; Abcd2, ATP-binding cassette, subfamily D member 2; Pmch, pro-melanin-concentrating hormone; Gad1, glutamic acid decarboxylase 1, Rac1, RAS-related C3 botulinum substrate 1; Wnt7a, wingless-related MMTV integration site 7A; Myo5a, myosin VA; Gal, galanin; Grin1, glutamate receptor, ionotropic, NMDA1; Slc6a4, solute carrier family 6 member 4; Cdkn1a, cyclin-dependent kinase inhibitor 1A; Fgfr3, fibroblast growth factor receptor 3; En2, engrailed 2; Gbx2, gastrulation brain homeobox 2. (c) PCR validation of differences in gene expression after CR in db/db mice.