Photoperiod-Dependent Effects of 4-tert-Octylphenol on Adherens and Gap Junction Proteins in Bank Vole Seminiferous Tubules

Figure 4

(a) Representative Western blot analysis of N-cadherin, β-catenin, and connexin 43 protein expression levels in homogenates of testis explants from bank voles kept in either long light cycles (18L : 6D; 𝑛=8) or short light cycles (6L : 18D; 𝑛=8). Explants were cultured in control medium (c) or in media containing 10, 100, or 500 mg/L 4-tert-octylphenol for 24 h. Anti-β-actin labeling served as an internal protein loading control. (b)–(d) The relative amount of N-cadherin (b), β-catenin (c), and connexin 43 (d) proteins normalized to β-actin. Data obtained from three separate analyses is expressed as mean ± SD. Significant differences from control values are denoted as ^∗𝑃<0.05 and ^∗∗𝑃<0.01.

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