Figure 2: MCH initiates the removal of some MCHR1 from the plasma membrane. BHK-570 cells were transfected with VSVg-tagged MCHR1. (a) Untreated cells and cells that were exposed to 1  M MCH for 30 min were fixed and immunostained with mouse anti-VSVg primary antibody and goat anti-mouse Alexa Fluor 488 secondary antibody. (b) The loss of MCHR1 from the plasma membrane in response to 1  M MCH was measured using a modified cell-based ELISA protocol. Background signal from mock-transfected cells was subtracted. (6 triplicate experiments). *denotes statistical significance at when compared to 100% control using Student’s -test.