Figure 5: VSVg-MCHR1-containing vesicles are distinct from those associated with -arrestins. VSVg-MCHR1 and GFP -arrestin 1-expressing cells (a) and VSVg-MCHR1 and GFP -arrestin 2-expressing cells (b) were treated with 1  M MCH for 30 min, then fixed, and immunostained with anti-VSVg antibody. Confocal microscopy was performed using a grid pattern confocal microscope and Image-Pro Plus software. A single slice is shown as an overlay of both Alexa Fluor (546 nm) and GFP (488 nm) signals.