Sperm Proteomics: Road to Male Fertility and Contraception

Table 1

Summary of major proteomic studies to define the fertile male (animal models).

Sample	Proteomic technique	Study outcome	Authors
Mouse spermatozoa	LC-MS/MS	Identification of 52 proteins following capacitation	Baker et al. [61]
Boar spermatozoa	LC-MS	Identification of proteins from the sperm surface that are responsible for binding of the sperm to the oocyte	Belleannee et al. [27]
Bull spermatozoa	LC-MS/MS	Identification of 131 proteins to differentiate normal and pyriform sperm	Shojaei Saadi et al. [62]
Bull spermatozoa (Holstein)	LC-MS/MS	Identification of proteins of impaired sperm function due to elevated testicular temperature, with important implications for fertility predictions	Newton et al. [63]
Accessory sex gland fluid (Holstein)	LC-MS/MS	Increased expression of bovine seminal plasma protein identified in high-fertility bulls	Moura et al. [64]
Accessory sex gland (dairy bull)	LC-MS/MS	Identification of isoforms of osteopontin and phospholipase A2 (PLA2) in high-fertility bulls	Moura et al. [65]
Mouse spermatozoa	MS/MS	Identification of 299 testis-specific proteins and detection of 155 novel proteins involved in spermiogenesis	Guo et al. [66]
Mouse spermatozoa	MS/MS	Identification of 100 proteins that are expressed on mature sperm at the site of sperm-oocyte interactions	Stein et al. [67]
Rat spermatozoa	LC-MS/MS	Identification of 5123 peptides from rat spermatozoa	Baker et al. [68]
Mouse spermatozoa	MS/MS	Identification of capacitation-associated changes in the phosphorylation status (55 in vivo sites) of mouse sperm proteins	Piatt et al. [69]
Mouse spermatozoa	PMF, LC-MS/MS	Identification of 100 proteins of capacitation	Nixon et al. [70]

LC-MS/MS: liquid chromatography-tandem mass spectrometry; LC-MS: liquid chromatography-mass spectrometry; MS/MS: tandem mass spectrometry; PMF: peptide mass fingerprints.