Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells

Figure 3

Effect of quercetin on pIRS-1 (ser307) expression. Quantitative analysis and representative western blot analysis of pIRS-1 (ser307) in HIT-T15 cells pretreated with quercetin and Gelam honey extract in cells cultured in 20 mM ((a), (c), (e)) and 50 mM ((b), (d), (f)) glucose. The results were normalized with β actin antibody. Data were presented as the mean ± standard deviation. (e) ^∗𝑃<0.05; ^#𝑃<0.005 quercetin and honey extract treated compared to the 20 mM glucose alone. (f) ^∗𝑃<0.05; ^#𝑃<0.005 quercetin and honey extract treated compared to the 50 mM glucose alone.

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