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International Journal of Endocrinology
Volume 2013, Article ID 367956, 5 pages
http://dx.doi.org/10.1155/2013/367956
Research Article

Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome

1The State Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
2Department of Obstetrics and Gynecology, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China
3The First Affiliated Hospital, Nanjing Medical University, China

Received 24 January 2013; Revised 23 April 2013; Accepted 9 May 2013

Academic Editor: Maria L. Dufau

Copyright © 2013 Xu Boqun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. We previously found that expression of SET gene was up-regulated in polycystic ovaries by using microarray. It suggested that SET may be an attractive candidate regulator involved in the pathophysiology of polycystic ovary syndrome (PCOS). In this study, expression and cellular localization of SET protein were investigated in human polycystic and normal ovaries. Method. Ovarian tissues, six normal ovaries and six polycystic ovaries, were collected during transsexual operation and surgical treatment with the signed consent form. The cellular localization of SET protein was observed by immunohistochemistry. The expression levels of SET protein were analyzed by Western Blot. Result. SET protein was expressed predominantly in the theca cells and oocytes of human ovarian follicles in both PCOS ovarian tissues and normal ovarian tissues. The level of SET protein expression in polycystic ovaries was triple higher than that in normal ovaries . Conclusion. SET was overexpressed in polycystic ovaries more than that in normal ovaries. Combined with its localization in theca cells, SET may participate in regulating ovarian androgen biosynthesis and the pathophysiology of hyperandrogenism in PCOS.