Figure 2: Example of calcium activity measurement. The figure depicts the trace of two cells obtained during a single experiment: a capsaicin-responsive (dark purple) and a capsaicin-unresponsive (light purple) neuron. The three small photomicrographs show the same neurons, at different stages of the experiment: (1) before stimulation, (2) during stimulation with 20 mM K+, and (3) during stimulation with 0.5 μM Capsaicin + algogenic chemicals. Black arrows beneath the trace indicate the beginning of stimulation. Time scale bar; 120 s. Cap; capsaicin.