Calcium Activity of Upper Thoracic Dorsal Root Ganglion Neurons in Zucker Diabetic Fatty Rats
Figure 4
Traces and box plots from capsaicin experiments. Panels (a), (d), (g), and (j) show traces (examples) of the time- development for control and diabetic dorsal root ganglion neurons. The arrows indicate the onset of 10 sec stimulation with (a) capsaicin, (d) combination of Capsaicin and algogenic chemicals (AC); (g) combination of 0.3 μM Capsaicin and AC at pH 6.0, and (j) 0.5 μM Capsaicin and AC at pH 6.0. The traces were background corrected. As a measure of , the vertical axis is shown as the 340/380 nm light intensity ratio. (a) Vertical scale: 0.10; horizontal scale: 20 s, (d) vertical scale: 0.20; horizontal scale: 20 s.; (g) vertical scale: 0.10; horizontal scale: 20 s., and (j) vertical scale: 0.20; horizontal scale: 20 sec. Panels (b), (e), (h), and (k) display box plots on the absolute area under the curve (AUC) derived from stimulation with the in (a), (d), (g), and (j) mentioned capsaicin-containing solutions. Panels (c), (f), (i), and (l) display box plots with median, 25th and 75th percentiles on the relative AUC of the capsaicin-containing solution-derived increase and the preceding 20 mM K+-derived increase. The error bars indicate the 10th and 90th percentiles. Values below the 10th and above the 90th percentiles are shown as dots. + indicates the mean. *.
