Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells

Figure 1

Effect of LPS on activin A secretion at various densities of AEC. Human AEC were seeded in 96-well microplates at densities of 1,250, 2,500, 5,000, 10,000, and 20,000 cells per well in 200 μL of DME/Ham’s F12 medium supplemented with 10% FBS, 100 μg/mL streptomycin, and 0.5 μg/mL amphotericin B. Either LPS (10 μg/mL) or vehicle (control) was added to each well. After 96 h of culture, activin A in the medium was measured. Closed rectangles: LPS. Open rectangles: control. The results from the quadruplicate assay are shown as the mean ± SE (𝑛=4). ^a𝑃<0.05 compared with the control value at 1,250 cells/well. ^b𝑃<0.01 compared with the control value at 1,250 cells/well. ^∗𝑃<0.01 compared with the control value at each cell density.