The ETS-Domain Transcription Factor Elk-1 Regulates COX-2 Gene Expression and Inhibits Glucose-Stimulated Insulin Secretion in the Pancreatic β-Cell Line INS-1
Figure 2
Elk-1 RNAi downregulated COX-2 gene expression. (a) INS-1 cells were transiently transfected with control siRNA and Elk-1 siRNAs (1, 2, and 3), respectively. Untransfected cells were used as control. 48 h after transfection, Elk-1 protein levels were assayed by Western blot analysis. β-actin levels served as internal control. (b and c) INS-1 cells were transiently transfected with control siRNA and Elk-1 siRNA-1, respectively. 48 h after transfection, Elk-1 and COX-2 mRNA levels were determined by quantitative real-time RT-PCR. Relative mRNA expression was expressed as mean ± SEM. * versus control siRNA transfected group. (d) Elk-1 and COX-2 protein levels were assayed by Western blot analysis. β-actin levels served as internal control.
