The ETS-Domain Transcription Factor Elk-1 Regulates COX-2 Gene Expression and Inhibits Glucose-Stimulated Insulin Secretion in the Pancreatic β-Cell Line INS-1
Figure 4
Elk-1 upregulated COX-2 promoter activity through Elk-1 binding site. The Elk-1 expression vector (pCMV3.0b-Elk-1) or control vector (pCMV3.0b) was transfected together with construct pCOX-2 (−2026/+44), pCOX-2 (−2026/+44, m−82/−69), or pCOX-2 (−2026/+44, del−82/−69). Relative luciferase activity was expressed as mean ± SEM and represented three different experiments in triplicate for each fragment. * versus control. # versus pCOX-2 (−2026/+44) transfacted with pCMV3.0b- Elk-1.
