Orexin A Affects INS-1 Rat Insulinoma Cell Proliferation via Orexin Receptor 1 and the AKT Signaling Pathway
Figure 2
Proliferation and viability of INS-1 cells treated with OXA. Cells were treated with OXA at concentrations of 0 M, 10−8 M, 10−10 M, and 10−6 M for 24 h. In addition, a separate group of cells was treated with 10−6 M OXA in the presence of the OX1R antagonist SB334867 (OX1Ri) (10−6 M) for 24 h. Proliferation and viability were determined by BrdU assays and the MTT test. Data are presented as mean SEM based on triplicate determinations from a representative experiment. Asterisks indicate significant differences compared to control (*).
