Orexin A Affects INS-1 Rat Insulinoma Cell Proliferation via Orexin Receptor 1 and the AKT Signaling Pathway
Figure 4
OXA increases INS-1 cell proliferation via the AKT signaling pathway. INS-1 cells were treated with OXA (10−6 M) for 20 min in the presence of PF-04691502 (AKTi) (10−6 M), wortmannin (PI3Ki) (10−8 M), SB334867 (OX1Ri) (10−6 M), or the combination of these antagonists. The phosphorylation of AKT (-AKT) (corresponds to 60 kDa) was normalized against the total protein (-AKT) activation. The -AKT protein expression was used as an internal control for equal protein loading. Protein activation was measured by western blot analysis. Data are presented as mean SEM based on triplicate determinations from a representative experiment. Asterisks indicate significant differences compared to control samples (*).
