Resveratrol Prevention of Diabetic Nephropathy is Associated with the Suppression of Renal Inflammation and Mesangial Cell Proliferation: Possible Roles of Akt/NF-B Pathway
Figure 2
Resveratrol (RSV) attenuated high glucose- (HG-) induced plasminogen activator inhibitor (PAI-1) expression and cell proliferation in rat mesangial cell (RMC), which might be Akt/nuclear factor-kappa B (NF-B) pathway dependent. RMCs were cultured in DMEM containing 5.6 mM glucose (NG) and 10% FBS. When the confluence reached at 60%–70%, the medium was replaced with NG and 0.2% BSA. 24 h later, the cells were pretreated with 10 M LY294002, 1 M MK-2206, or an equal volume of DMSO for 30 min and then incubated for another 24 h with or without HG (25 mM) in the presence or absence of RSV (25 M). After that, cells were collected and protein levels of PAI-1 ((a), (b)), Akt, p-Akt ((c), (d)), and NF-B ((e), (f)) were detected using Western blotting assay. Additionally, cell proliferation was examined with CCK8 assay and BrdU incorporation, respectively. Results represent as mean . compared with NG, compared with HG.
